Summary
Type: SPE
Application #: 302210
Catalog#: 730079 - SPE Cartridges, Chromabond, SB, 3ml, PP, 500mg, 50/pk
Detection: The fractions were analysed by GC-FID and HPLC. The system was equipped with a 150 mm x 4 mm i.d. Nucleosil C18-PAH (100 A, 5µm) column and an 8 mm x 4 mm i.d. Nucleosil C18 (120 A, 5µm) guard column.
Conditions: Column pretreatment: The SPE colums were prepared by filling 0,7g Chromabond SB and 2,0g of silicia gel (inactivated by 10% water (w:w)) between three PTFE frits.
Column conditioning: with n-hexane
Elution: PAHs (polycyclicaromatic hydrocarbons), PASHs and PAOHs (containing sulphur and oxygen) were eluted with 3ml n-hexane, 12ml n-hexane / dichloromethane (85:15;v:v) and 2ml dichloromethane (fraction 1). Subsequently PANHs (containing nitrogen) and neutral metabolites were eluted with 1 ml dichloromethane, 6ml methanol and 3 ml 0,05 N hydrochloric acid in methanol (fraction 2).
Authors: Stelmach B A Müller A Weiler E W
Source: Chemosphere 40 (2000) 359-367